Production of Bioflocculant through Fermentation of Spoilt Orange Juice with Bacillus spp Isolated from Sediment of Local Clay Pot

The biodegradable and non-toxic Bioflocculants have attracted considerable interest as alternative to non-biodegradable chemical flocculants. However, the cost of fermentation media for bioflocculant production low flocculation efficiency is a major challenge. In this study, we grow Bacillus spp isolated from sediment local clay pot on spoilt orange juice produce bioflocculant. culture supernatant bacilli grown were screen using Jar test method Kaolin suspension model wastewater. effect pH temperature also studied. cations bioflocculation rate produced tested via hybridization with cations. sample wastewaters determined. initially had between 31.1±1.6 % 76.4±1.2% when cultured screening media. Bioflocculation peaked 77.9% at 40 oC while lowest (55.41%) was obtained 25 oC. optimum (about 78%) recorded 6 – 7 highest (84.2%) achieved 96th hour incubation. Na+ Fe3+ serious inhibitory K+, Ca2+, Mg2+ Al3+ less or no up 77.7% 64.5 Kitchen wastewater aquaculture This study reveals potential utilization fermentable substrate especially if conditions parameters are well optimized.